X-RAY MICROSCOPY OF BIOLOGICAL SPECIMENS
نویسندگان
چکیده
منابع مشابه
X-ray contact microscopy system for spectromicroscopy of biological specimens.
An X-ray contact microscopy system has been developed for the study of molecular and elemental distributions in biological specimens based on X-ray absorption characteristics. The system consists of a chamber for measuring XANES (X-ray absorption near-edge structure) of biomolecules, and a contact microscopy system for dried specimens with an electronic zooming tube. With this system the elemen...
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X-ray scanning analytical microscopy (XSAM) can be used to visualize the elemental distribution in biological specimens. In this study, the authors prepared standard specimens for XSAM and performed quantitative analysis of various elements dissolved in soft tissues. Two different types of standard specimens were prepared. Methylmethacrylate (MMA) resin-based standard specimens were prepared wi...
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Biological specimens were observed using a soft X-ray microscope constructed at the Ritsumeikan synchrotron radiation center BL12. The spatial resolution of the microscope using an X-ray wavelength of 2.4nm was estimated to be 75nm from the edge response. Three kinds of diatoms were observed and ultrastructures of approximately 100nm could be clearly resolved. Two kinds of bacteria and red bloo...
متن کاملIdeal standards for quantitative x-ray microanalysis of biological specimens.
The use of ion exchange beads is proposed as standards for the estimation of various elements in biological tissues. Elements to be detected or quantitated in biological tissues occur in three forms: as free ions or salts, in a crystalline phase, and bound to a matrix. For the latter category a standard system using ChelexlOO -jon exchange beads is proposed. Cations can be attached in a control...
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Soft-x-ray cryotomography allows quantitative and high-resolution three-dimensional imaging of intact unstained cells. To date, the method relies on synchrotron-radiation sources, which limits accessibility for researchers. Here we present a laboratory water-window microscope for cryotomography. It is based on a λ=2.48 nm liquid-jet laser-plasma source, a normal-incidence multilayer condenser, ...
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ژورنال
عنوان ژورنال: Le Journal de Physique Colloques
سال: 1978
ISSN: 0449-1947
DOI: 10.1051/jphyscol:1978423